Fig. 4.

Retinol and ascorbate enhance reprogramming of epiblast stem cells to naïve pluripotency. (A) Experimental setup for EpiSC reprogramming experiments. (B) Oct4:GFP⁺ colonies following 6–10 d of reprogramming in VitA+/− 2i/LIF media. (Left) Representative microscopic field views at day 10 (4× magnification; the GFP channel is colored green and superimposed over a brightfield image of the same view). (Right) Frequency of reprogrammed colonies (± SD; n = 3). (C) Frequency of Oct4:GFP⁺ colonies after 6 d of reprogramming in VitA− 2i/LIF media with supplemented retinol and ascorbate (± SD; n = 3).