Fig. S3.

Retinol does not affect TET function posttranslation. (A) Global levels of 5mC (Left) and 5hmC (Right) in 2i/LIF-grown TET-TKO ESCs that were partially rescued by forced expression of TET1 (dark gray) and exposed to retinol for 72 h. Similar data were observed for TET2 (Fig. 2D). (± SD; n = 3). (B) Relative activity of TET1-CD when supplemented with 100 μM iron (Fe²⁺ or Fe³⁺) and retinol or RA, compared with ascorbate, and controls such as DMSO (carrier) or no reducing agent (NR). (± SD; n = 3).