Skip to main content
. 2016 Oct 10;113(43):12262–12267. doi: 10.1073/pnas.1608147113

Fig. 5.

Fig. 5.

S1/S2 cleavage facilitates early virus entry. (A) Calu3 and Huh7 cells were incubated with protease inhibitors [100 µM camostat (camo), 50 µM PCI, 10 µM E64d], or with DMSO, and then transduced with MERS or VSV pps. (B) Three HAE cultures were incubated with indicated protease inhibitors, and then transduced with WT and S1/S2 mutant MERS pps. (C) Huh7 and Huh7/TMPRSS2 cells were incubated with protease inhibitors, and then transduced with WT or S1/S2 mutant (YSAS or SSVR) MERS pps. Virus entry was quantified by measuring luciferase levels in infected cells at 48-h (A, C) or 18-h (B) posttransduction. Error bars present SD from the mean (n = 3). Statistical significance was assessed by Student's t test. *P < 0.05; †P < 0.01; ‡P < 0.001; ns, not significant.