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. 2016 Oct 10;113(43):12150–12155. doi: 10.1073/pnas.1613055113

Fig. S6.

Fig. S6.

Drug-induced translation arrest within the leader ORFs of CHL resistance genes. Toeprinting analysis of drug-dependent ribosome stalling within the leader ORFs of (A–C) cat86AL and (D) cmlAL CHL resistance genes. All of the reactions loaded onto gels shown in A–D contained mupirocin, an inhibitor of Ile-tRNA synthetase. In all of the panels, the site of CHL-induced arrest is indicated by a black triangle, the “mupirocin band” is indicated by a gray triangle, and the start codon is marked by a black dot. (A) CHL-dependent arrest of translation of the cat86AL ORF driven by Bacillus subtilis ribosomes. (B and C) Oxazolidinone antibiotics (B) tedizolid (Tzd) and (C) LZD stall the E. coli ribosome within the cat86AL gene at the site of programmed CHL-induced arrest. (D) LZD arrests the E. coli ribosome at the site of CHL-induced arrest within the cmlAL leader ORF.