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. 2016 Oct 10;113(43):12150–12155. doi: 10.1073/pnas.1613055113

Table S1.

DNA primers used in the study

Primer name Sequence
Ala -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTGCAAC TCTGAACAAC ATCCGTAC
Arg -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTCGAAC TCTGAACAAC ATCCGTAC
Asn -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTAACAC TCTGAACAAC ATCCGTAC
Asp -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTGATAC TCTGAACAAC ATCCGTAC
Cys -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTTGCAC TCTGAACAAC ATCCGTAC
Gln -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTCAGAC TCTGAACAAC ATCCGTAC
Glu -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTGAAAC TCTGAACAAC ATCCGTAC
Gly -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTGGCAC TCTGAACAAC ATCCGTAC
His -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTCATAC TCTGAACAAC ATCCGTAC
Ile -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTATTAC TCTGAACAAC ATCCGTAC
Leu -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTCTTAC TCTGAACAAC ATCCGTAC
Lys -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTAAAAC TCTGAACAAC ATCCGTAC
Met -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTATGAC TCTGAACAAC ATCCGTAC
Phe -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTTTTAC TCTGAACAAC ATCCGTAC
Pro -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTCCGAC TCTGAACAAC ATCCGTAC
Ser -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTAGCAC TCTGAACAAC ATCCGTAC
Thr -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTACCAT TCTGAACAAC ATCCGTAC
Trp -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTTGGAC TCTGAACAAC ATCCGTAC
Tyr -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTTATAC TCTGAACAAC ATCCGTAC
Val -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTGTGAC TCTGAACAAC ATCCGTAC
hns-FL-fwd ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CACTTAAAAC TCTGAACAAC ATCCGTACTC TTC
hns-S-fwd ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGGAAG CACTTAAAAC TCTGAACAAC ATCCGTACTC TTC
hns-SE-fwd ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGG CACTTAAAAC TCTGAACAAC ATCCGTACTC TTC
hns-SEA-fwd ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
hns-SEAL-fwd ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAAAAC TCTGAACAAC ATCCGTACTC TTC
hns-SEALK-fwd ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAC TCTGAACAAC ATCCGTACTC TTC
hns-rev-trunc GGT TAT AAT GAA TTT TGC TTA TTA ACC TTG CCT GCG CAC GAA GAG TAC GGA TGT TGT TCA GAG T
T7 ATTAATACGACTCACTATAGGG
NV1 GGTTATAATGAATTTTGCTTATTAAC
Arg -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAACGACTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Asn -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAAACCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Asp -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAGATCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Cys -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAATGCCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Gln -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAACAGCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Glu -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAGAACTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Gly -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAGGCCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
His -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAACATCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Ile -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAATTCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Leu -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAACTTCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Lys -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAAAACTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Met -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAATGCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Phe -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAATTTCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Pro -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAACCGCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Ser -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAAGCCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Thr -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAACCCTTAAAAT TCTGAACAAC ATCCGTACTC TTC
Trp -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAATGGCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Tyr -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAATATCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Val -FL-1 ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAGTGCTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Ala -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CAGCAAAAAC TCTGAACAAC ATCCGTACTC TTC
Arg -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CACGAAAAAC TCTGAACAAC ATCCGTACTC TTC
Asn -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CAAACAAAAC TCTGAACAAC ATCCGTACTC TTC
Asp -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CAGATAAAAC TCTGAACAAC ATCCGTACTC TTC
Cys -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CATGCAAAAC TCTGAACAAC ATCCGTACTC TTC
Gln -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CACAGAAAAC TCTGAACAAC ATCCGTACTC TTC
Glu -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CAGAAAAAAC TCTGAACAAC ATCCGTACTC TTC
Gly -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CAGGCAAAAC TCTGAACAAC ATCCGTACTC TTC
His -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CACATAAAAC TCTGAACAAC ATCCGTACTC TTC
Ile -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CAATTAAAAC TCTGAACAAC ATCCGTACTC TTC
Lys -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CAAAAAAAAC TCTGAACAAC ATCCGTACTC TTC
Met -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CAATGAAAAC TCTGAACAAC ATCCGTACTC TTC
Phe -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CATTTAAAAC TCTGAACAAC ATCCGTACTC TTC
Pro -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CACCGAAAAC TCTGAACAAC ATCCGTACTC TTC
Ser -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CAAGCAAAAC TCTGAACAAC ATCCGTACTC TTC
Thr -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CAACCAAAAT TCTGAACAAC ATCCGTACTC TTC
Trp -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CATGGAAAAC TCTGAACAAC ATCCGTACTC TTC
Tyr -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CATATAAAAC TCTGAACAAC ATCCGTACTC TTC
Val -FL-P ATTAATACGACTCACTATA GGGATATA AGGAGGA AAACAT ATGAGCGAAG CAGTGAAAAC TCTGAACAAC ATCCGTACTC TTC
T7-cmlaL TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGAGCACTTCCAAGAACGCAGACAAGGTCTGCGTTC
T7-cmlaL-2A TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGGCCACTTCCAAGAACGCAGACAAGGTCTGCGTTC
T7-cmlaL-3A TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGAGCGCTTCCAAGAACGCAGACAAGGTCTGCGTTC
T7-cmlaL-4A TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGAGCACTGCCAAGAACGCAGACAAGGTCTGCGTTC
T7-cmlaL-5A TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGAGCACTTCCGCGAACGCAGACAAGGTCTGCGTTC
T7-cmlaL-6A TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGAGCACTTCCAAGGCCGCAGACAAGGTCTGCGTTC
T7-cmlaL-7A TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGAGCACTTCCAAGAACTCAGACAAGGTCTGCGTTC
T7-cmlaL-8A TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGAGCACTTCCAAGAACGCAGCCAAGGTCTGCGTTC
T7-cmlaL-9A TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGAGCACTTCCAAGAACGCAGACGCGGTCTGCGTTC
cmaL-toe2 CGTAGAGCAAAAGGATTCATGAGAACGCCGCAACAACCGAAAAATGAAGGTTGCTGCGGCTTACTTGTCTGCGTTCTTG
cmaL-toe2-6A CGTAGAGCAAAAGGATTCATGAGAACGCCGCAACAACCGAAAAATGAAGGTTGCTGCGGCTTACTTGTCTGCGGCC
cmaL-toe2-7A CGTAGAGCAAAAGGATTCATGAGAACGCCGCAACAACCGAAAAATGAAGGTTGCTGCGGCTTACTTGTCTGAGTTC
cmaL-toe2-8A CGTAGAGCAAAAGGATTCATGAGAACGCCGCAACAACCGAAAAATGAAGGTTGCTGCGGCTTACTTGGCTGCGTTC
cmaL-toe2-9A CGTAGAGCAAAAGGATTCATGAGAACGCCGCAACAACCGAAAAATGAAGGTTGCTGCGGCTTACGCGTCTGCGTTC
rev-cmlaL-toe2 CGTAGAGCAAAAGGATTCATG
T7-catL TAATACGACTCACTATAGGGGGACAGAAACATGACATATCTCTTGAAAGGATGATTGTGGTGGTGAAAACAGATAAAATCTCCTCC
T7-catL-2A TAATACGACTCACTATAGGGGGACAGAAACATGACATATCTCTTGAAAGGATGATTGTGGTGGCGAAAACAGATAAAATCTCCTCC
T7-catL-3A TAATACGACTCACTATAGGGGGACAGAAACATGACATATCTCTTGAAAGGATGATTGTGGTGGTGGCAACAGATAAAATCTCCTCC
T7-catL-4A TAATACGACTCACTATAGGGGGACAGAAACATGACATATCTCTTGAAAGGATGATTGTGGTGGTGAAAGCAGATAAAATCTCCTCC
T7-catL-5A TAATACGACTCACTATAGGGGGACAGAAACATGACATATCTCTTGAAAGGATGATTGTGGTGGTGAAAACAGCTAAAATCTCCTCC
T7-catL-6A TAATACGACTCACTATAGGGGGACAGAAACATGACATATCTCTTGAAAGGATGATTGTGGTGGTGAAAACAGATGCAATCTCCTCC
catl-toe1 CAGATAATTTTCGTCTATTTGTTTAAACAATTTTATCTCCTCCTGAATGTGATTTACTGTATTCAGGAGGAGATTTTATCTGTTTTCACC
catl-toe1-2A CAGATAATTTTCGTCTATTTGTTTAAACAATTTTATCTCCTCCTGAATGTGATTTACTGTATTCAGGAGGAGATTTTATCTGTTTTCGC
catl-toe1-3A CAGATAATTTTCGTCTATTTGTTTAAACAATTTTATCTCCTCCTGAATGTGATTTACTGTATTCAGGAGGAGATTTTATCTGTTGCCAC
catl-toe1-4A CAGATAATTTTCGTCTATTTGTTTAAACAATTTTATCTCCTCCTGAATGTGATTTACTGTATTCAGGAGGAGATTTTATCTGCTTTCAC
catl-toe1-5A CAGATAATTTTCGTCTATTTGTTTAAACAATTTTATCTCCTCCTGAATGTGATTTACTGTATTCAGGAGGAGATTTTAGCTGTTTTCAC
catl-toe1-6A CAGATAATTTTCGTCTATTTGTTTAAACAATTTTATCTCCTCCTGAATGTGATTTACTGTATTCAGGAGGAGATTGCATCTGTTTTCAC
rev-cat-toe1 CAGATAATTTTCGTCTATTTGTTTAAAC
T7-cat-2Al-V TAATACGACTCACTATAGGGGGACAGAAACATGACATATCTCTTGAAAGGATGATTGTGGTGAAAGCAGATAAAATCTCCTCC
T7-catl-2A-VK TAATACGACTCACTATAGGGGGACAGAAACATGACATATCTCTTGAAAGGATGATTGTGGTGGCAGATAAAATCTCCTCC
T7-catl-2A-VKA TAATACGACTCACTATAGGGGGACAGAAACATGACATATCTCTTGAAAGGATGATTGTGGTGGATAAAATCTCCTCC
catl-toe1-2A-V CAGATAATTTTCGTCTATTTGTTTAAACAATTTTATCTCCTCCTGAATGTGATTTACTGTATTCAGGAGGAGATTTTATCTGCTTTCAC
catl-toe1-2A-VK CAGATAATTTTCGTCTATTTGTTTAAACAATTTTATCTCCTCCTGAATGTGATTTACTGTATTCAGGAGGAGATTTTATCTGCCACCAC
catl-toe1-2A-VKA CAGATAATTTTCGTCTATTTGTTTAAACAATTTTATCTCCTCCTGAATGTGATTTACTGTATTCAGGAGGAGATTTTATCCACCACAAT
T7-cmlal-S TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGACTTCCAAGAACGCAGACAAGGCGATCTAAGC
T7-cmlal-ST TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGTCCAAGAACGCAGACAAGGCGATCTAAGC
T7-cmlal-STS TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGAAGAACGCAGACAAGGCGATCTAAGC
T7-cmlal-STSK TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGAACGCAGACAAGGCGATCTAAGC
T7-cmlal-STSKN TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGGCAGACAAGGCGATCTAAGC
T7-cmlal-STSKNA TAATACGACTCACTATAGGGCGATTCAAATTCAATCATGAGATAGTCAGCAGATGGACAAGGCGATCTAAGC
cmlaL-STSKN-toe2 CGTAGAGCAAAAGGATTCATGAGAACGCCGCAACAACCGAAAAATGAAGGTTGCTGCGGCTTAGATCGCCTTGTCTGCC
cmlaL-STSKNA-toe2 CGTAGAGCAAAAGGATTCATGAGAACGCCGCAACAACCGAAAAATGAAGGTTGCTGCGGCTTAGATCGCCTTGTCCATC
Ala -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTGCAAC TCTGAACAAC ATCCGTAC
Arg -FL-A GATATA AGGAGGA AAACAT ATGAGCGAAGCACTTCGAAC TCTGAACAAC ATCCGTAC