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. 2016 Oct 4;113(43):E6639–E6648. doi: 10.1073/pnas.1606050113

Fig. 1.

Fig. 1.

Exponentially increasing dosing schedules during priming durably increase antigen-specific IgG production relative to traditional bolus immunization. Groups of C57BL/6 mice (n = 5 per group) were immunized with 5 µg gp120 mixed with 25 µg MPLA according to the dosing schedules shown in A, followed by a single bolus booster injection of 5 µg gp120 + 25 µg MPLA on day 21. (B) Fold change in antibody concentration on day 14 postprime relative to bolus injection. *P < 0.05; ***P < 0.0001 compared with bolus injection determined by ANOVA with Dunnett’s test post hoc using bolus injection as the control. Shown are means ± SEM. (C) Total serum anti-gp120 IgG as measured by ELISA. Asterisk indicates statistically different from boost d21 group as determined by two-way ANOVA with Dunnett’s post hoc test using bolus injection as the control. Data are representative of two independent experiments.