Fig. 2.
Determination of tissue-specific variation in mt-tRNA incorporation into the mammalian mt-LSU. Selected tissues and cultured cells isolated from (A) pig (heart, liver, skeletal muscle, and dermal fibroblasts), (B) human (osteoblasts and embryonic kidney cells), (C) macaque (heart and liver), and (D) rat (heart, liver, skeletal muscle, brain, and kidney) were analyzed to determine any tissue-specific variation in mt-tRNA incorporation into the mt-LSU. Upon lysis, the samples were sedimented through sucrose gradients, followed by RNA extraction. RNA samples from fractions 1 and 2 (free pool) and fractions 10 and 11 (mt-LSU-associated) were subjected to Northern blotting. Abundance of mt-tRNAPhe (green) and mt-tRNAVal (orange) in free (pale green/orange), and mt-LSU-associated fractions (dark green/orange) is represented as a percentage of their combined signal.