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. 2016 Oct 7;113(43):12076–12081. doi: 10.1073/pnas.1605086113

Fig. 5.

Fig. 5.

Optimal target concentrations strongly depend on assumed initial KD distribution. The plot shows the mean KD as a measure of pool binding strength for the SELEX pool at cycle 20 using different constant target concentration. Depending on the initial distribution of ligands, we find vastly different optimal target concentrations, i.e., concentrations with lower mean KD.