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Exocytosis of NR1 is mediated by SNARE complexes. (A) Exocytosis of pH-NR1 was inhibited by the treatment of Botox C, but not BotoxB. (B–D) SNARE proteins mediate the exocytosis of pH-NR1. Scramble shRNA (Scr), the shRNA of each SNARE protein [SNAP25 (B), VAMP1 (C), syntaxin4 (STX4) (D)], or the shRNA-resistant SNARE protein was coexpressed with pH-NR1 in dissociated hippocampal neurons. *P < 0.05; **P < 0.01. n.s., no statistical significance.
