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MS of isolated DAB. (A) NSI-MS of DAB was performed as described in SI Materials and Methods using deuteroheme and (trace i) (12C-methyl) SAM, (trace ii) (13C-methyl) SAM, or (trace iii) (d3-methyl) SAM as substrates. (B) The MS/MS of DAB or (C) 13C-methylated DAB revealed multiple fragments when using CID at 40%. Predicted fragment structures are shown in Fig. S4.
