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MS following solvent-derived deuterium incorporation into DAB. ChuW was assayed in buffered H2O (trace i) and 70% buffered D2O (trace ii). DAB was isolated as described in SI Materials and Methods and analyzed by NSI-MS. When assayed in buffered D2O, we observed a 43% enrichment of the A+1 peak at 528.27 m/z for the product (trace ii). NSI-MS of isolated DAB in d4-methanol (trace iii).
