Fig. 2.

ICP27 promotes use of cryptic 5′ splice sites. (A and B) Read counts mapping to ZER1 (negative strand; A) and DESI2 (positive strand; B). Previously described transcript variants (thick black lines denote exons) are shown below. Arrows denote differences in intron 1 between ICP27 and control-transfected cells. (C) RT-PCR for ZER1 and DESI2 of HSV-1–infected and mutant virus-infected (Fig. 1E) HEK293 cells. Cryptic 5′ splice sites were confirmed by sequencing of RT-PCR products and are illustrated in red. The same set of cDNAs were used for both Left and Right. NIC, noninfected control.