Fig. 3.

ICP27-induced retention of specific introns in some host genes. (A–C) Read counts mapping to POLR2A (last intron retention; A), NFS1 (intron 1 retention; B), and ATXN2L (multiple internal intron retention; C). Arrows indicate significant differences in read counts. (D) Schematic diagrams of inserts in HSV-2 ICP27 expression plasmids. (E) Effect of ICP27 mutations in HSV-1 viruses (Fig. 1E) and HSV-2 plasmids on intron retention of cellular genes by RT-PCR of infected (8 hpi) or transfected HEK293 cells. Arrows denote RT-PCR primers (Table S2). Exons are numbered in boxes. NIC, noninfected control.