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. 2016 Oct 10;113(43):12256–12261. doi: 10.1073/pnas.1609695113

Fig. S2.

Fig. S2.

ICP27 can inhibit the splicing of both a first short intron and a second intron, facilitating use of a PAS located in the second intron. (A) Read counts mapping to a representative ICP27-targeted gene, EIF4G3, eukaryotic translation initiation factor 4 gamma 3 (shown from 3′ to 5′ because it is expressed from the negative strand of chromosome 1). A close-up of the impacted region is shown in Lower. A downward-facing arrow shows the reads that are significantly different in ICP27-transfected cells compared with control. Reads mapping to the intronic PAS are also labeled in the close-up. (B) RT-PCR study of splicing inhibition of introns 1 and 2 and use of the PAS mapping to intron 2 of EIF4G3 by ICP27 in HSV-1 infected cells. cDNAs were obtained from 293 cells infected with HSV wild-type or ICP27 mutant viruses (as indicated) at a MOI of 3 and at 5 hpi. RT-PCR primers are shown using arrows in the diagram to the right.