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. 2016 Oct 10;113(43):12256–12261. doi: 10.1073/pnas.1609695113

Fig. S7.

Fig. S7.

Cotranscriptional splicing of β-globin pre-mRNA is not inhibited by ICP27. The 293 cells were transfected with a β-globin expression plasmid (pβ-globin), ICP34.5 expression plasmid (pICP34.5-full), and KSHV K8 expression plasmid (pST1) together with the HSV-1 ICP27 expression plasmid (pHSV-1 ICP27), the HSV-2 ICP27 expression plasmid (pICP27), or pFlag vector. cDNAs were prepared from the total RNAs extracted from transfected cells with or without reverse transcription. RT-PCRs were performed by using specific primers (Table S2) using the same cDNA prepared from the total RNAs extracted from transfected cells.