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. 2016 Oct 10;113(43):E6610–E6619. doi: 10.1073/pnas.1605350113

Fig. 4.

Fig. 4.

V-1 overexpression reduces the cortical content of the Arp2/3 complex. (A–D) Representative examples of a control cell (A; CTL), a cell overexpressing a high level of mRFP-V-1 [B; OE V-1 (H)), a cell overexpressing a low level of mRFP-V-1 [C; OE V-1 (L)), and a cell overexpressing a high level of mRFP-FBM V1 [D; OE FBM V-1 (H)) that had been stained for F-actin by using Alexa Fluor 647-labeled phalloidin (F-actin) and an antibody to D.d. Arp3 (Arp3). Also shown is the merge between these two signals (Merge), the signal for mRFP-V-1 or mRFP-FBM V-1, and the transmitted light image (DIC). All fluorescence images are maximum-intensity projections of complete optical sections. (Scale bar, 10 µm.) (E) The ratio of Arp3 fluorescence to F-actin fluorescence within 1 µm of the plasma membrane at protruding edges (excluding filopodia) for control cells (CTL), cells expressing L, M, and H levels of mRFP-V-1, and cells overexpressing high levels of mRFP-FBM V-1 (OE FBM V-1 (H)). Mean and SD values are as follows: CTL, 0.88 ± 0.18; L, 0.79 ± 0.16; M, 0.54 ± 0.17; H, 0.41 ± 0.12; OE FBM V-1 (H), 0.90 ± 0.20. Additional statistically different values are as follows: L vs. M, P < 0.05; L vs. H, P < 0.01; M vs. H, P < 0.05. Note that V-1–null cells exhibited a value of 0.82 ± 0.15, which was not significantly different from CTL. **P < 0.01; ***P < 0.001.