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. 2016 Oct 10;113(43):E6610–E6619. doi: 10.1073/pnas.1605350113

Fig. 9.

Fig. 9.

V-1–null cells exhibit defects in chemotactic aggregation. (A–D) Representative examples of streaming assays performed using WT cells (A; WT), V-1–null cells (B; V-1 Null), V-1–null cells expressing FLAG-V-1 (C; V-1 Null + V-1), and V-1–null cells expressing FLAG-FBM V-1 (D; V-1 Null + FBM V-1) imaged at 6 h (Upper) and 12 h (Lower) with bright field. (Scale bar, 100 µm.) (E–G) Representative 15-min path plots (taken from phase images) for ripple-stage WT cells (E) and V-1–null cells (F), and the migration speeds of ripple-stage WT cells, V-1–null cells, V-1–null cells expressing FLAG-V-1, and V-1–null cells expressing FLAG-FBM V-1 (G). (Scale bar, 50 μm.) Mean and SD values (in µm/min) are as follows: WT, 11.58 ± 3.32; V-1–null, 5.52 ± 2.56; V-1–null + V-1, 10.2 ± 3.28; V-1–null + FBM V-1, 5.58 ± 1.94. ***P < 0.001.