Effect of FLV depletion on photosystems efficiencies upon light fluctuations. Dark-acclimated moss tissues grown for 10 d in control light were then exposed to cycles of 5 min of mild actinic illumination (50 µmol photons m−2⋅s−1, orange bars) and 1 min strong actinic light (540 µmol photons m−2⋅s−1, yellow bars and shading), monitoring PSII and PSI efficiencies simultaneously. QA relative reduction and Y(NA) parameter are reported in A and B, whereas additional parameters are shown in SI Appendix, Fig. S11. WT, flva KO, and flvb KO lines are shown in black, red, and blue, respectively. Data reported are average ± SD of three independent replicates, and asterisks indicate values significantly different between WT and flv KOs (t test, *P = 0.05; **P = 0.01).