Fig. 6.

LRV1 elimination results in decreased release of cytokines from infected macrophages. TNF-α or IL-6 levels were quantified 24 h after infection of macrophages with Lbr LEM2780 (A) or Lg M4147 (B) parent, GFP65 knockdown control, or LRV1-StL transfectants. In both studies, the LRV1-control was Lgy M4147. For A, results are averages of two to three technical replicates for two clones of each line. For B, results are the averages of two technical replicates for three to six clones of each line. NS, not significant; **P < 0.01; ***P < 0.0001 by t test.