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qPCR analysis of LRV1 RNA levels in LRV1 StL clones of L. braziliensis strain LEM2780 (A), L. braziliensis strain LEM3874 (B), and recloned L. guyanensis M4147 RDRP StL c3 (C). White bars denote a nonspecific product; black bars denote an LRV1-specific product (melt curve analysis). Dashed line indicates cutoff for designating a clone as LRV1-negative. Error bars are the SD of three technical replicates for each line.
