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. 2016 Oct 18;113(43):11998–12005. doi: 10.1073/pnas.1615085113

Table S3.

Primer sequences used to amplify regions of LRV1 for cloning into stem-loop (StL) constructs

Parasite strain Target Construct ID Name Stem length, bp Sequence*
Lbr LEM2700 Capsid B6910 pIR2HYG-LRV1_LbrLEM2700_CapsidStL(A) 943 5′-CGCTAGTCTAGAATACTACAGCAAACATGTTTCG
5′-CGCTAGTCTAGACAAGGTGTCTGTTGGGTTCGAT
RDRP B6908 pIR2HYG-LRV1_LbrLEM2700_RDRPStL(A) 1,143 5′-CGCTAGTCTAGAATGTGCTTCAAACTTGAAGATG
5′-CGCTAGTCTAGATAGCAGCAATCTAACGACCTGC
Lbr LEM2780 Capsid B7061 pIR2HYG-LRV1_LbrLEM2780_CapsidStL(A) 835 5′-CCAGCTTGGGATCAATTTGCGG
5′-GGACATCTCCATCAGCCGATGA
RDRP B7062 pIR2HYG-LRV1_LbrLEM2780_RDRPStL(A) 794 5′-GTGAGGATGAGTTGCGCGCTGC
5′-ATTGCTAAGTAGACTGTTTGCG
Lbr LEM3874 Capsid/RDRP B7268 pIR2HYG-LRV1_LbrLEM3874_StL(A) 1,000 5′-GGCTAGTCTAGA GTCGTGCGATCTATTCCATCCT
5′-GGCTAGTCTAGATTAGTGCTTATGTTAGGATCAG
Lgy M4147 Capsid B7066 pIR2HYG-LRV1_LgyM4147_CapsidStL(A) 926 5′-CTTCTCCTTTACGTGCCAGC
5′-GCGCATTGTTGTCCACTCAA
RDRP B7063 pIR2HYG-LRV1_LgyM4147_RDRPStL(A) 829 5′-CTTGCTAGGTCGTGGGGTGA
5′-ACCAACATGCATAGACGTGG