Table S3.
Primer sequences used to amplify regions of LRV1 for cloning into stem-loop (StL) constructs
| Parasite strain | Target | Construct ID | Name | Stem length, bp | Sequence* |
| Lbr LEM2700 | Capsid | B6910 | pIR2HYG-LRV1_LbrLEM2700_CapsidStL(A) | 943 | 5′-CGCTAGTCTAGAATACTACAGCAAACATGTTTCG |
| 5′-CGCTAGTCTAGACAAGGTGTCTGTTGGGTTCGAT | |||||
| RDRP | B6908 | pIR2HYG-LRV1_LbrLEM2700_RDRPStL(A) | 1,143 | 5′-CGCTAGTCTAGAATGTGCTTCAAACTTGAAGATG | |
| 5′-CGCTAGTCTAGATAGCAGCAATCTAACGACCTGC | |||||
| Lbr LEM2780 | Capsid | B7061 | pIR2HYG-LRV1_LbrLEM2780_CapsidStL(A) | 835 | 5′-CCAGCTTGGGATCAATTTGCGG |
| 5′-GGACATCTCCATCAGCCGATGA | |||||
| RDRP | B7062 | pIR2HYG-LRV1_LbrLEM2780_RDRPStL(A) | 794 | 5′-GTGAGGATGAGTTGCGCGCTGC | |
| 5′-ATTGCTAAGTAGACTGTTTGCG | |||||
| Lbr LEM3874 | Capsid/RDRP | B7268 | pIR2HYG-LRV1_LbrLEM3874_StL(A) | 1,000 | 5′-GGCTAGTCTAGA GTCGTGCGATCTATTCCATCCT |
| 5′-GGCTAGTCTAGATTAGTGCTTATGTTAGGATCAG | |||||
| Lgy M4147 | Capsid | B7066 | pIR2HYG-LRV1_LgyM4147_CapsidStL(A) | 926 | 5′-CTTCTCCTTTACGTGCCAGC |
| 5′-GCGCATTGTTGTCCACTCAA | |||||
| RDRP | B7063 | pIR2HYG-LRV1_LgyM4147_RDRPStL(A) | 829 | 5′-CTTGCTAGGTCGTGGGGTGA | |
| 5′-ACCAACATGCATAGACGTGG |