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Cloning strategy for the fusion proteins. (A) The template gene sequence was constructed with the following order: fimH-csgA-papG-fimH-csgA linked with a GAAGCGGC GGCGAAA sequence repeated five times. (B) Generation of the fusion genes from the combination of specific primers for each gene. Dimeric and trimeric genes are abbreviated with the first letter of the fimH (f), csgA (c), and papG (p) genes.
