Figure 3.

Generation of the fusion proteins. (A) The specific primers were combined to amplify the fusion genes by PCR, cloned in the pLATE31 plasmid and verified by colony PCR. (B) Fusion genes cloned into pLATE31 were used to transform BL21 (DE3) E. coli, followed by induction with 1 mM IPTG and purification of the 6His-tagged fusion proteins by Ni-NTA affinity chromatography. (C) Western blot assays with anti-6His (C-Term) HRP antibodies were employed to verify the fusion. Line 1 corresponds to FimH (1040 bp and 29.5 kDa), line 2 to CsgA (539 bp and 11.9 kDa), line 3 to PapG (1139 bp and 33.9 kDa), line 4 to FC (1442 bp and 44.9 kDa), and line 5 to FCP (2444 bp and 82.1 kDa). MW, molecular weight. bp, base pairs.