Figure 1. Comparative biosensor behavior in response to HMGB-1 (4 μg/mL) in buffer.

Both experiments A and B were carried out under the same conditions and prepared using the alkanethiol/EDC/NHS surface onto which the HMGB-1 monoclonal capture antibodies were covalently bound (amine coupling). Experiment (a) resulted in a low specificity signal of ΔR = 3% ± 0.08; while experiment (b) gave a lower and inconsistent response of ΔR = 1.5% ± 0.12.