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. 2016 Oct 31;6:36146. doi: 10.1038/srep36146

Figure 3. MicroRNA-214-3p (miR-214-3p) negatively modulates MEF2C expression.

Figure 3

(A) Verification of MEF2C as a target gene of miR-214-3p by the dual luciferase reporter system. Predicted miR-214-3p seed matches to the sequence in the 3′-UTR of MEF2C gene mRNA. The seed sequence of miR-214-3p is UGGAAUG, and the complementary nucleotide sequences are shown in red words. Data are shown as mean ± sem, **p < 0.01 vs pGl3-promoter vector control, N = 3. (B) MEF2C mRNA and protein expression in the myocardium of Ang-II-infusion mouse model of cardiac hypertrophy were assessed by RT-qPCR assay and western blot assay, respectively. Data are shown as mean ± sem, *p < 0.05, **p < 0.01, ***p < 0.001. N = 5-8. MEF2C mRNA and protein expression in Ang-II-induced mouse cardiomyocytes (C) and mouse cardiomyocytes transfected with miR-214-3p mimic or MEF2C siRNA (D). Data are shown as mean ± sem, *p < 0.05, **p < 0.01. N = 3. (E) MEF2C mRNA and protein expression in the myocardium of Ang-II-infusion mouse model of cardiac hypertrophy with overexpression of miR-214-3p. Data are shown as mean ± sem, **p < 0.01. N = 6.