Figure 5. Up-regulation of microRNA-214-3p (miR-214-3p) in NMVCs through the NF-κB pathway.

(A) Time-dependent activation of NF-κB signaling in Ang-II-treated NMVCs. MiR-214-3p expression in Ang-II-induced NMVCs with knockdown of P65 (B), or with pre-treatment with the NF-κB inhibitor JSH23 or QNZ, respectively (C), or with overexpression of IKB-α (D) was assessed by RT-qPCR assay. Additionally, miR-214-3p expression in NMVCs with overexpression of IKK-β was also detected (E). Data are shown as mean ± sem, *p < 0.05, **p < 0.01, ***p < 0.001. N = 3.