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. 2016 Oct 31;20:34. doi: 10.1186/s40824-016-0081-3

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of DOTAP lipid on properties of lipid-PLGA nanoparticles (LPHNPs) for gene delivery application. a Schematic diagram for LPHNPs-DNA complex formation and transfection, b TEM-image of bare PLGA NPs, c TEM-image of LPHNPs, d Effect of DOTAP concentration on LPHNPs surface charge, e Effect of DOTAP concentration on LPHNPs transfection efficiency in various cells (Flow cytometry analysis) and f fluorescence microscopy. (Reproduced from reference.15)