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. Author manuscript; available in PMC: 2017 Sep 21.
Published in final edited form as: J Am Chem Soc. 2016 Sep 12;138(37):12021–12024. doi: 10.1021/jacs.6b06566

Table 1.

Binding Free Energy and Affinity Determined by ITC at 10 °C

L99A L99A/M102Q
ΔG (kcal/mol) Ka (×104 M−1) ΔG (kcal/mol) Ka (×104 M−1)
Bnz −5.54 ± 0.04 1.89 ± 0.12 −5.96 ± 0.04 3.95 ± 0.29
Ab −5.04 ± 0.03 0.77 ± 0.05 −6.40 ± 0.02 8.77 ± 0.28
EtBnz −5.54 ± 0.06 1.91 ± 0.18 −5.37 ± 0.03 1.41 ± 0.07
BEtAb −5.12 ± 0.02 0.90 ± 0.04 −5.59 ± 0.02 2.08 ± 0.06