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GUCY2C-specific CARs mediate antigen-dependent T cell activation. 1 × 106 CAR-expressing T cells were stimulated for 6 h with plate-coated antigen (BSA or GUCY2C) or PMA and Ionomycin (PMA/IONO). T cell activation markers CD25 (A) and CD69 (B) were quantified by flow cytometry. Histograms are gated on live CD8+GFP+ T cells. Results are representative of three experiments.
