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. 2016 Sep 2;5(10):e1227897. doi: 10.1080/2162402X.2016.1227897

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — Real-time GUCY2C-specific CAR T cell-mediated cytotoxicity. CT26 (A and C) or CT26.GUCY2C (B and D) mouse colorectal cancer cells were seeded at 10,000 cells/well in an E-Plate and CAR-T cells, media, or Triton-X 100 (Triton) were added to the plate 24 h later (time = 0). The E-Plate was scanned every 15 min to quantify relative electrical impedance (normalized to time = 0). (A and B) Solid lines indicate the mean of duplicate wells and surrounding clouds indicate standard deviation. Results are representative of two experiments. (C and D) % specific lysis values for each CAR-T cell and target cell combination were calculated from the impedance data at the indicated time points. All statistical tests in (C) and (D) compared (1) Control to MS7, (2) Control to MS24, and (3) MS7 to MS24 (**p < 0.01, ****p < 0.0001, Two-way ANOVA). (C and D) show the means ± standard deviation of two experiments.