Figure 3.

Oral microbiota do not influence 4-NQO induced oral cancer progression in mice. (A) Oral microbiota in mouse tongue from CD24^+/− and CD24^−/− mice were analyzed by pyrosequencing. Distribution of predominant bacterial family and species (>0.1%) were presented. Data shown is the Mean ± SD, n = 3 for each group. (B, C) Wild-type C57BL/6J mice were housed in GF (germ-free) or SPF (specific pathogen free) conditions. Both groups were subjected to 50 μg/mL 4-NQO for 16 weeks then switched to regular drinking water. (B) Mouse body weight change was monitored (SPF n = 14, GF, n = 18). (C) Total tumor burden of mice were quantified based on the combined factors including number of tongue lesions, primary lesion size, number of oral cavity lesions, primary oral cavity lesion size, and tumor diagnosis (p = 0.22 by two-tailed Student's t-test).