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. 2016 Aug 12;5(10):e1219826. doi: 10.1080/2162402X.2016.1219826

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Melanoma-conditioned medium (CM) prevents endothelial cell (EC) death under severe hypoxia. (a) HUVEC and (b) HMVEC survival under hypoxia upon treatment with melanoma CMs collected under normoxic conditions. (c) HUVEC and (d) HMVEC survival under hypoxia upon treatment with melanoma CMs collected under hypoxic conditions. Subconfluent ECs were treated with various melanoma CMs (BLM: ; M14: ; Mel57: ; 530: ; 1F6: ; Mel57-VEGF¹⁶⁵: ) and basal medium (). Cells were fixed at 24, 48, and 72 h and the percentage of total surviving cells was determined. Normalized cell viability (%) data in (a–d) are expressed relative to treatment with basal medium (DMEM + 10% FBS) at 24 h normoxia and represent mean ± SEM of three independent experiments, conducted in triplicate. *p < 0.05. (e) Morphology of ECs under various treatments at 48 h hypoxia. Image in the dotted black box shows EC morphology with EC culture medium under normoxic conditions. Scale bar, 100 µm.

Inline graphic — Melanoma-conditioned medium (CM) prevents endothelial cell (EC) death under severe hypoxia. (a) HUVEC and (b) HMVEC survival under hypoxia upon treatment with melanoma CMs collected under normoxic conditions. (c) HUVEC and (d) HMVEC survival under hypoxia upon treatment with melanoma CMs collected under hypoxic conditions. Subconfluent ECs were treated with various melanoma CMs (BLM: ; M14: ; Mel57: ; 530: ; 1F6: ; Mel57-VEGF¹⁶⁵: ) and basal medium (). Cells were fixed at 24, 48, and 72 h and the percentage of total surviving cells was determined. Normalized cell viability (%) data in (a–d) are expressed relative to treatment with basal medium (DMEM + 10% FBS) at 24 h normoxia and represent mean ± SEM of three independent experiments, conducted in triplicate. *p < 0.05. (e) Morphology of ECs under various treatments at 48 h hypoxia. Image in the dotted black box shows EC morphology with EC culture medium under normoxic conditions. Scale bar, 100 µm.