Figure 2.

Macrophage depletion and iNOS blockade impairs T cell infiltration and RT5 tumor rejection 24 week-old Rip1-Tag5 (RT5) mice were injected with 25 μg of CpG-ODN 1668 and activated TCR8 CD8⁺ T cells as described in Fig. 1. (a) Kaplan-Meier survival curves. (b) Quantification by cytofluorometry of infiltrating leukocyte subpopulations in RT5 tumors. (c) Representative immunofluorescence microscopy analysis of tumor vessels of the indicated groups of mice stained with antibodies against CD31 (red) and VCAM-1 (green). Yellow color shows colocalization. Size bar indicates 100 μm. (d) Quantification of VCAM-1 expression on CD31⁺ tumor vessels. Tumors of comparable size were investigated. Three-6 random sections were analyzed from each tumor. (e) Protein concentration of IFNγ, CCL5, CXCL9 and CXCL10 in tumor lysates as determined by Multiplex analysis. Results are shown as mean ±SEM of 10 mice per group from two independent experiments. *p < 0 .05, ** p < 0 .01, ***p < 0 .001, ns = not significant.