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. 2016 Sep 20;5(10):e1226718. doi: 10.1080/2162402X.2016.1226718

Figure 2.

Figure 2.

Effect of sorafenib treatment on ERK phosphorylation levels and absolute number of EPC. Pre-treatment samples of PBMC from HCC patients were treated with different concentrations of sorafenib (0–20 μM) in vitro and (A) the number of pERK+ EPC and (C) the absolute number of EPC were measured as described in Materials and Methods section. Each symbol (Inline graphic) represents an individual HCC patient and lines represent mean values for the group. (B) The number of pERK+ EPC/mL and (D) absolute number of EPC in HCC patients before (▪) or after treatment (•) with sorafenib (in vivo) was measured as described in the materials and methods.