Figure 8.

Characterization of the tumor-infiltrating immune cells. MCA 205 tumors were implanted in C57BL/6 (n = 5) and treated as described in Fig. 6. Four days after the last injection of WR, tumors were processed for flow cytometry determination of the number of CD3⁺, CD4⁺, and CD8⁺ T cells (A). The ratio of CD8⁺ T cells to Treg cells (B) and the number of CD8⁺ IFNγ⁺ TNFα⁺ T cells (C) is also shown. The expression of the immune checkpoint PD-1 was also determined (D). *p < 0.05 by Mann-Whitney test.