FIG. 4.

Distribution of mitochondria in 2-cell embryos cultured with increasing concentrations of P_i. These MPLSM micrographs illustrate the mitochondrial organization in 2-cell embryos labeled with Mitotracker and cultured for 5.5 h in HECM-10 containing different concentrations of P_i: A) 0 µM, B) 1.25 µM, C) 2.5 µM, D) 5.0 µM, and E) a second image of the same embryo shown in A collected following the imaging of all other embryos in all other treatments. All embryos shown are from the same replicate; bar = 25 µm. F) The graph shows the quantitation of the mitochondrial distribution (see Materials and Methods for explanation of quantitation). *Significantly different from control (P < 0.01).