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. Author manuscript; available in PMC: 2016 Oct 31.
Published in final edited form as: Biol Reprod. 2001 Jun;64(6):1845–1854. doi: 10.1095/biolreprod64.6.1845

FIG. 6.

FIG. 6

Mitochondria distribution in embryos permitted to recover from pHi perturbation. The first row of TPLSM images (Time 1: A–E) shows the distribution of mitochondria in embryos cultured for 4 h in either control medium (HECM-10) or either HTMA or HDMO. Embryos were rinsed, stained, placed into the second culture treatment (HECM-10, HTMA, or HDMO), then imaged. Time 2 is 3 h after Time 1, when embryos were imaged a second time (F–J). Immediately after the second imaging, the embryos were fixed, stained for actin, and imaged with LSCM (K–O). Abbreviation in front of slash mark is initial culture medium; abbreviation after slash mark is second culture medium. Scale bar = 25 µm. P) The distribution of mitochondria was quantified and the results are shown in this graph. Data include three replicates. n = 12 embryos for all treatments except HECM/HECM Time 2 where n = 10 embryos. *Significant difference (P < 0.05) from control (HECM/HECM). ●Significant difference (P < 0.05) of treated embryos rinsed and placed into HECM-10 from their respective negative control (either HTMA/HTMA or HDMO/HDMO).