TABLE 1.
The pHi, and development of 2-embryos cultured with increasing concentrations of DMO and TMA.
| Compound concentrationa |
pHib | % Morula and blastocyst |
% Blastocyst |
|---|---|---|---|
| TMA 0 mM | 7.186 ± 0.04 | 97.3 | 73.3 |
| TMA 5 mM | 7.285 ± 0.07 | 82.6c | 34.7d |
| TMA 10 mM | 7.383 ± 0.04c | 49.1d | 6.0d |
| TMA 20 mM | 7.421 ± 0.06c | 5.17d | 2.6d |
| TMA 40 mM | 7.538 ± 0.04d | 0d | 0d |
| TMA 80 mM | 7.685 ± 0.05d | 0d | 0d |
| DMO 0 mM | 7.242 ± 0.04 | 90.58 | 74.7 |
| DMO 5 mM | 7.221 ± 0.07 | 87.1 | 61.1c |
| DMO 10 mM | 7.09 ± 0.05c | 46.3d | 21.6d |
| DMO 20 mM | 6.87 ± 0.05d | 0d | 0d |
TMA series consisted of 115 embryos per treatment over 14 replicates. DMO series consisted of 170 embryos per treatment over 7 replicates.
Determined after 4 h culture; n > 20 embryos per treatment.
Significantly different from control at P < 0.05.
Significantly different from control at P < 0.01.