TABLE 2.
Nucleus and blastomere size in 2-cell embryos cultured in media containing pHi-altering compounds.
| HECM-10 (n)a | HTMA (n) | HDMO (n) | |
|---|---|---|---|
| A. Ratio of nuclear diameter to blastomere diameter (N/B ± SEM) | |||
| Fixed embryos | |||
| 3 h | 0.35 ± 0.005 (23) | 0.30 ± 0.005b (21) | 0.33 ± 0.007 (14) |
| 6 h | 0.31 ± 0.004 (46) | 0.27 ± 0.004b (52) | 0.31 ± 0.002 (40) |
| Live embryos | |||
| 0 h | 0.30 ± 0.004 (17) | 0.31 ± 0.003 (18) | 0.31 ± 0.004 (18) |
| 3 h | 0.32 ± 0.004 (18) | 0.29 ± 0.003b (18) | 0.33 ± 0.004b (18) |
| 6 h | 0.32 ± 0.003 (16) | 0.29 ± 0.004b (18) | 0.33 ± 0.003 (17) |
| B. Actual nuclear and blastomere diameters in live embryos (µm ± SEM) | |||
| Nuclear diameter | |||
| 0 h | 17.79 ± 0.26 (17) | 17.71 ± 0.17 (18) | 17.84 ± 0.22 (18) |
| 3 h | 17.98 ± 0.19 (18) | 17.29 ± 0.23b (18) | 18.19 ± 0.23 (18) |
| 6 h | 18.24 ± 0.20 (16) | 17.22 ± 0.29b (18) | 18.36 ± 0.14 (17) |
| Blastomere diameter | |||
| 0 h | 58.45 ± 0.53 (17) | 57.76 ± 0.41 (18) | 57.99 ± 0.45 (18) |
| 3 h | 56.85 ± 0.32 (18) | 58.99 ± 0.39b (18) | 55.09 ± 0.34b (18) |
| 6 h | 57.01 ± 0.37 (16) | 60.03 ± 0.62b (18) | 55.23 ± 0.40b (17) |
a
(n) = number of embryos.
b
Significantly different at P < 0.05 from control within time point.