Table 1.
BE% (Equation (6)) for the biosensor designs PtC/PoPD/PEI2/GluOx5/XL fabricated using a range of crosslinkers (XLs) at different concentrations and crosslinking conditions determined on day 0 and day 10. ΔBE% (day 0) is the difference in the day-0 BE% value compared with the crosslinker-free design. The 10-day stability value was calculated as the change in BE% on day 10 compared with that of day 0 for each biosensor design. The results for PEGDE crosslinked at room temperature or at 55 °C were statistically the same, and were, therefore, pooled.
| Crosslinker | BE% (Day 0) | BE% (Day 10) | ΔBE% (Day 0) | p-Value | 10-Day Stability | p-Value |
|---|---|---|---|---|---|---|
| none | 41 ± 2 (n = 16) | 26 ± 3 (n = 14) | 0 | N/A | −15 ± 4 | <0.001 |
| GA (1%) | 37 ± 1 (n = 2) | 23 ± 2 (n = 2) | −4 ± 2 | >0.61 | −14 ± 3 | <0.03 |
| GA (25%) | 39 ± 3 (n = 8) | 45 ± 3 (n = 6) | −3 ± 4 | >0.57 | +6 ± 4 | >0.19 |
| PEG (0.1%) | 45 ± 2 (n = 3) | 19 ± 6 (n = 3) | +4 ± 3 | >0.41 | −26 ± 5 | <0.02 |
| PEG (1%) | 45 ± 8 (n = 3) | 21 ± 6 (n = 3) | +4 ± 7 | >0.46 | −24 ± 9 | <0.07 |
| PEGDE (0.1%) | 39 ± 2 (n = 7) | 34 ± 3 (n = 7) | −2 ± 3 | >0.55 | −5 ± 4 | >0.28 |
| PEGDE (1%) | 34 ± 2 (n = 12) | 35 ± 3 (n = 12) | −7 ± 3 | <0.05 | +1 ± 4 | >0.78 |