. 2016 Oct 22;16(10):1760. doi: 10.3390/s16101760

Table 2.

Binding parameters calculated for α, β, γ and ω fluorescence maxima of RAW, PARAFAC and NMF data under increasing Zn concentrations.

	α-Site RAW						α-Site PARAFAC						α-Site NMF
HAs No.	EEWP (nm/nm)	I_ML a.u	logK	R	f %	C_L (mmol/g)	EEWP (nm/nm)	I_ML a.u	logK	R	f %	C_L (mmol/g)	EEWP (nm/nm)	I_ML a.u	logK	R	f %	C_L (mmol/g)
HA1	275/500	112	4.11	1.00	60	2.44	275/520	91	4.41	0.99	58	3.85	280/515	62	4.14	0.95	69	0.25
HA2	270/495	139	3.76	1.00	49	0.38	275/510	92	3.75	1.00	56	0.44	505/275	73	4.22	0.63	61	0.25
HA3	275/490	n/a					270/515	103	4.32	0.87	22	12.5	270/515	71	5.91	0.67	25	10.5
HA4	270/490	86	3.71	0.99	38	2.17	270/505	65	3.52	0.98	43	0.60	275/505	48	4.16	0.34	40	0.41
HA5	270/505	182	4.07	0.99	59	1.44	265/525	184	4.19	1.00	60	1.63	280/525	127	4.37	0.91	52	3.89
	β-Site RAW						β-Site PARAFAC						β-Site NMF
HAs No.	EEWP (nm/nm)	I_ML a.u	logK	R	f %	C_L (mmol/g)	EEWP (nm/nm)	I_ML	logK	R	f %	C_L (mmol/g)	EEWP (nm/nm)	I_ML	logK	R	f %	C_L (mmol/g)
HA1	360/500	75	4.15	1.00	64	1.57	360/520	60	4.31	1.00	65	2.05	360/515	45	4.29	0.96	72	0.25
HA2	365/495	94	3.88	1.00	50	0.25	365/510	70	3.88	1.00	56	0.25	365/505	61	4.17	0.85	58	0.25
HA3	365/490	114	5.08	0.92	13	17.8	365/515	77	4.02	0.96	24	8.00	370/515	59	4.36	0.76	25	8.48
HA4	360/490	32	3.87	0.99	53	1.40	365/505	43	3.73	0.99	42	0.86	370/505	34	4.06	0.61	42	0.25
HA5	n/d						n/d						n/d
	γ-Site RAW						γ-Site PARAFAC						γ-Site NMF
HAs No.	EEWP (nm/nm)	I_ML a.u	logK	R	f %	C_L (mmol/g)	EEWP (nm/nm)	I_ML a.u	logK	R	f %	C_L (mmol/g)	EEWP (nm/nm)	I_ML a.u	logK	R	f %	C_L (mmol/g)
HA1	435/510	56	4.13	1.00	70	1.50	445/520	56	4.22	1.00	67	2.33	445/515	51	4.10	1.00	69	1.38
HA2	435/505	53	3.97	1.00	67	0.62	440/510	51	3.96	1.00	67	0.68	440/505	50	3.93	1.00	67	0.65
HA3	n/d						430/515	56	3.77	0.96	30	4.87	435/515	56	4.10	0.96	27	8.10
HA4	435/505	32	3.87	0.99	53	1.40	440/505	33	3.83	0.99	52	1.29	440/505	31	3.76	0.99	53	0.71
HA5	445/525	29	4.03	1.00	69	0.85	450/525	27	4.00	1.00	69	0.85	455/525	28	4.07	1.00	66	1.70
	ω-Site RAW						ω-Site PARAFAC						ω-Site NMF
HAs No.	EEWP (nm/nm)	I_ML a.u	logK	R	f %	C_L (mmol/g)	EEWP (nm/nm)	I_ML a.u	logK	R	f %	C_L (mmol/g)	EEWP (nm/nm)	I_ML a.u	logK	R	f %	C_L (mmol/g)
HA1	n.d.						275/450	85	3.97	1.00	51	2.41	275/450	85	3.92	0.99	52	2.87
HA2	n.d.						275/435	134	3.79	0.99	29	7.97	270/440	145	4.09	0.93	26	11.02
HA3	n.d.						275/440	n/a					275/445	n/a
HA4	n.d.						270/430	71	3.77	0.99	25	6.77	270/440	84	6.88	0.92	16	11.71
HA5	n.d.						260/455	44	3.84	0.99	51	0.59	260/470	48	4.01	0.96	55	1.29

n/a: not applicable, model cannot be applied; n/d: no data, fluorescence maximum was not found.