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. 2016 Jun 17;2(7):e89. doi: 10.1097/TXD.0000000000000605

FIGURE 5.

FIGURE 5

Changes in degranulation (CD107a surface expression) and IFNγ release upon coculture with different target cells. Analyses are performed on CD56dimNKG2A− NK cells. Full lines depict CMV group (n = 40/27 for K562/MRC-5, respectively), dotted lines depict control group (n = 18/15 for K562/MRC-5 respectively). A and C, Increased degranulation (A) and IFNγ release (C) on coculture with the tumor target cell line K562 after CMV infection compared with the control group. B and D, Increased degranulation (B) and IFNγ release (D) on coculture with the CMV-infected fibroblast cell line MRC-5 after CMV infection. Black lines show netto effect (activation after coculture with uninfected MRC-5 cells has been subtracted). Grey lines show NK cell activation after coculture with uninfected MRC-5 cells. E and F) No difference after CMV infection between single NK cell subpopulations in degranulation (E) and IFNγ release (F) upon coculture with the tumor target cell line K562; increased function can be observed in all subpopulations (exception: no increase in IFNγ release in the CD57+G2C− subset after CMV infection).