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. Author manuscript; available in PMC: 2016 Oct 31.
Published in final edited form as: Clin Cancer Res. 2015 Aug 13;21(24):5499–5510. doi: 10.1158/1078-0432.CCR-14-3091

Figure 5. MEK-inhibition induces p21 upregulation in p53 proficient CRC cells.

Figure 5

A. CRC cell lines were treated with DMSO (Control), 50 nM PD901, 50 nM MLN0128 or the combination of both for 16 hours. p21 mRNA levels were analyzed by qRT-PCR, normalized to GUSB mRNA levels and expressed as fold change compared to control. B. CRC cell lines were treated with DMSO (Control), 50 nM PD901, 50 nM MLN0128 or the combination of both for 24 hours and whole-cell protein extracts were analyzed by Western blotting with the indicated antibodies. GAPDH antibody was used as loading control. Figures are representative of three independent experiments.