Figure 3. Cluster Activity Dynamics Under Different Behavior States.

A. Representative cluster activities during maximum velocity (V_max) of locomotion. Upper panel: a representative mouse locomotion velocity peak; Lower panel: cluster activity of C1 and C4 (corresponding to clusters shown in Figure 2B). Vertical dash line indicated peak position of V_max B. Cross correlation between cluster C1 and C4 activity with V_max. Vertical dash line indicated zero time lag in the cross correlation. C. Quantification of cross-correlation between cluster activity and V_max for D1- or D2- MSN from Day One. Each dot indicated the average result from one mouse on Day One, and histogram bars indicated the mean value for all D1- or D2- mice, with error bars representing sem. D. Reliability of cross-correlation over 5 days, X-axis represented days, Y-axis represented averaged cross correlation value for each day, each dot indicating the averaged value from all D1- or D2- mice on the corresponding day, and error bars representing sem. E. Left panels were representative maps for cluster activity change from a D1-Cre (top left panel) and D2-Cre mouse (bottom left panel). Orientation of the map: M, medial; L, Lateral; R, Rostral; C, Caudal. Colored contour indicated the estimated boundary for each cluster. Dots with the same color as the contour indicate the centroids of neurons in the cluster. Gray dots indicated un-clustered neurons. Range indicator on the left indicated activity change, with reduced activity towards black color, and increased activity towards brighter red (D1-MSN) or green (D2-MSN). Horizontal bar beneath panels indicated the approximate location of dorsomedial and dorsolateral striatum (DMS and DLS). Scale bar 100 µm. Right panels were quantifications of cluster activity change near DMS (Blue color) and DLS (Pink color). Y-axis represented activity change, with negative value representing activity suppression and positive value representing activity increase. Each dot indicated value from one cluster. Black horizontal line represented the average value from all clusters in either DMS or DLS, error bar represented sem. Cocaine: activity change following cocaine injection; Saline: activity change following saline injection; AM/FM: activity difference between ambulation and fine movement. F. Quantification of cluster synchrony change near DMS (Blue color) and DLS (Pink color). Left panels were intra-cluster synchrony change for D1-MSN (top) and D2-MSN (bottom), Right panels were inter-cluster synchrony change for D1-MSN (top) and D2-MSN (bottom). The definition for positive and negative values in Y-axis was the same as E. Each dot indicated value from one cluster. Black horizontal line represented the average value from all clusters in either DMS or DLS, error bar represented sem. Asterisk (*) represents statistical significance.