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. 2016 Oct 1;143(19):3514–3521. doi: 10.1242/dev.137398

Fig. 3.

Fig. 3.

Effects of cyclopamine on gene expression and the AER. (A,B) Cyclopamine was applied at HH20/21 and Ptch1 expression was undetectable after 4 h (n=3/3) compared with untreated buds, but detectable at 72 h in cartilage condensations. (C,D) Shh expression increased at 4 h but reduced or undetectable at 24 h after cyclopamine treatment (n=11/22 reduced; 11/22 undetectable) and after this was undetectable. (E,F) Fgf8 expression expanded around the posterior margin of cyclopamine-treated buds by 24 h (red asterisks; n=7/7). (G) Hand-plate width was significantly greater in cyclopamine-treated wings at 48 h. (H) Hand-plate length was shorter in treated wings at 96 h. (I) The AER was longer in treated wings at 24 h. Error bars indicate s.e. (unpaired t-test, **P≤0.005, ***P≤0.0005, ****P≤0.0001). Scale bars: 500 μm (4 h, 24 h), 350 μm (48 h), 200 μm (72 h, 96 h).