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Shh represses the formation of a digit from the polarizing region. Grafted HH20 Gfp-expressing polarizing regions (A) contribute to the posterior margin of digit 3 in normal wings (n=9/9) in whole mounts showing GFP fluorescence (B), and serial sections showing Gfp (C) and Sox9 (D) expression. In HH20/21 cyclopamine-treated wing buds (E), the polarizing region forms a digit (n=3/3, F-H). Scale bars: 500 μm.
