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. 2016 Oct 1;143(19):3514–3521. doi: 10.1242/dev.137398

Fig. 8.

Fig. 8.

AER length is proportional to polarizing region digit number. (A) Weak Shh expression in the E11.5 mouse fore-limb bud polarizing region (red asterisks) completely overlaid by AER as observed by Fgf8 expression (black asterisk indicates limit of the AER, n=6/6). (B) Shh expression in the HH25 chick leg bud polarizing region partially overlaid by the Fgf8-expressing AER (n=8/8). (C) Shh expression in the HH26 chick wing bud polarizing region not overlaid by the Fgf8-expressing AER (n=8/8). (D) Shh expression is undetectable in the HH26 wing bud polarizing region treated with cyclopamine at HH19 slightly overlaid by the Fgf8-expressing AER (n=4/4). (E) Shh expression is undetectable in the HH26 wing bud polarizing region treated with cyclopamine at HH20/21 partially overlaid by the Fgf8-expressing AER (n=13/13). (F) Shh expression is weak in the HH26 chick wing bud polarizing region treated with cyclopamine at HH22/23 not overlaid by the Fgf8-expressing AER (n=5/5). Note the position of polarizing region cells that would have expressed Shh in D and E estimated by the shape of wing buds compared with normal wing buds. Scale bars: 350 μm.