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. 2016 Oct 1;143(19):3470–3480. doi: 10.1242/dev.134098

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Expression pattern of intestinal sox9 is conserved from lamprey to medaka. (A) Transverse section of lamprey larval intestine stained with H&E, highlighting the typhlosole as a single fold. (B) In situ detection of Sox9 expression at the base of the typhlosole. (C) EdU⁺ cells (24 h after injection) reveal basal proliferation zone (green; nuclei: DAPI, blue). (D-F) Medaka Sox9b:eGFP-expressing cells mark proliferative intestinal cells at the base of furrows. Confocal images of transverse cryosections of sox9b:gfp transgenic intestine at 10 dpf (D) in 3-week-old juveniles (E) and 8-week-old adult fish (F). Arrows indicate position of sox9b:eGFP-expressing cells at the base of folds. (G) Colocalization of endogenous sox9b expression domain shown by in situ hybridization and proliferation in EdU⁺ cells. (H) Colocalization of sox9b:eGFP-expressing cells and EdU staining. Arrows indicate sox9b:eGFP-expressing cells. Note that sox9b:eGFP-expressing cells are EdU⁺. (I) Sox9b endogenous expression shown by in situ hybridization in Gaudí^RSG-ubiquitin:^ERT2Cre transgenic fish (left panel), clonal analysis of Gaudí^RSG-ubiquitin:^ERT2Cre transgenic fish 2 weeks after induction. At the base of the furrow, proliferating GFP⁺ stem cells (middle panel) express sox9b (right panel). Scale bars: 100 µm (A-C); 25 µm (D,G,H,I); 50 µm (E,F). BV, blood vessel; I, intestine; T, typhlosole.