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. 2016 Oct 15;143(20):3817–3825. doi: 10.1242/dev.138941

Fig. 2.

Fig. 2.

Axonal projections of M71-, M72- and MOR23-expressing OSNs in a late Nrp1 conditional deletion context. (A-D) Dorsal and medial whole-mount views of X-Gal-stained Nrp1+/+;Omp+/cre;M71+/lacZ (P34) or Nrp1+/+;Omp+/cre;M72lacZ/lacZ (P40) OBs. A, anterior; L, lateral; P, posterior; M, medial; D, dorsal; V, ventral. Scale bar in A is 1 mm (scale is identical for all dorsal OB views). Note that the glomeruli are independent. Scale bar in B is 1 mm (scale is identical for all medial OB views). (E) Medial whole-mount view of an Nrp1+/+;Omp+/cre;MOR23GFP/GFP (P28) OB under epifluorescence. (F-I) Dorsal and medial whole-mount views of X-Gal-stained Nrp1flox/flox;Omp+/cre;M71+/lacZ (P36) or Nrp1flox/flox;Omp+/cre;M72lacZ/lacZ (P32) OBs showing a dorsalization of the glomeruli. (J) Medial whole-mount view of an Nrp1flox/flox;Omp+/cre;MOR23GFP/GFP (P28) OB under epifluorescence, showing no obvious targeting alterations. (K,M) Comparison of the frequency of the specific phenotypes affecting M71 or M72 glomeruli assigned to each of the following categories: independent (indep.), linked or fused glomeruli. The frequencies are compared between controls and late-deleted Nrp1 (*P<0.001, χ2 test). In addition, the presence or absence of an anterior glomerulus in each OB (control and late-deleted Nrp1) is indicated. (L,N,O) Mapping of all M71, M72 and MOR23 glomeruli (control and Nrp1 late-deleted populations) on a medial representation of the OB, showing the dorsalization of the M71 and M72 glomeruli. Glomeruli (from single OBs) linked to each other are connected by colored lines. A dot circled in black represents the mean position of glomeruli from each population. Ellipses represent the glomerular dispersion of each glomerular population. The dorsal part of the snout was considered as the horizontal line for all medial OB images, which corresponds to a 113° angle with the cribriform plate (L). Data corresponding to control animals (n=20 for M71, n=28 for M72 and n=12 for MOR23) are depicted in orange, and data from Nrp1 late-deleted animals (Nrp1flox/flox;Omp+/cre; n=10 for M71, n=22 for M72 and n=20 for MOR23) are in magenta. Insets show close-ups from the glomerular targeting sites.