Skip to main content
. 2016 Oct 15;143(20):3661–3673. doi: 10.1242/dev.138537

Fig. 1.

Fig. 1.

Deletion of Ptpn11 causes truncation of the mesencephalon at E12.5 and loss of the inferior colliculus at birth. (A,B) X-gal histochemistry on sagittal mouse brain sections. The bracket demarcates the tectal region that is lost in Ptpn1-cKO embryos. (C) Quantification of the length of the dorsal mesencephalon (mean±s.e.m.). Two-tailed unpaired t-test with Welch's correction. (D,E) Immunofluorescence for neurofilament (Nefl) and calbindin (Calb2). Dotted lines delimit cell and fiber stratification patterns. Nomenclature based on Edwards et al. (1986). (F,G) Nissl staining of sagittal sections of brains of the indicated genotypes at P25. (H-K) Neurogranin (Nrgn) staining of the inferior colliculus (ic) (outlined by dashed line) on sagittal sections of brains at P15. Nuclei are stained with Hoechst 33342. Boxed areas in H and I are enlarged in J and K, respectively; arrowheads indicate isolated Nrgn+ cells in the superior colliculus (sc). cb, cerebellum; PAG, periaqueductal gray; SAP, stratum album profundum; SGI, stratum griseum intermedium; SGP, stratum griseum profundum; SGS, stratum griseum superficiale; SO, stratum opticum.